Narrated by Barry (Tom Green) as he gives a tour on the campus of Ithaca College in upstate New York, “Road Trip” tells the story of his friend Josh (Breckin Meyer), Josh's girlfriend Tiffany (Rachel Blanchard), and Josh's new girlfriend Beth (Amy Smart). Josh and Tiffany were a couple and best friends since they were little kids. Their relationship became long-distance when they went away to different colleges.
Josh was misled to believe that Tiffany was cheating on him. He then slept with Beth, who videotaped the whole thing. At the same time Josh learns that Tiffany was not cheating on him, he discovers that the videotape had accidentally been sent to her. He then gathers a few friends who rush down to Austin, Texas in order to intercept the tape before Tiffany can see it. The story follows the boys on their adventurous trip to the University of Texas.